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Correlation between MLCK-FIP FRET/GFP ratio and RLC-P/total myosin ratio
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The distribution of RLC phosphorylation was assessed by determining the ratio of phosphorylated RLC to total myosin (right) and displayed according to the ratio bar (bottom right). Myosin with highly phosphorylated RLC is indicated in red. MLCK-FIP localization and activity are shown in the left panel. Arrows highlight regions of lamellipodia where high kinase activity colocalizes with myosin spots.